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· 資料簡介： CALCULATION OF RESULTS 1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (X) axis versus the corresponding concentration on the horizontal (Y) axis. 2. First, calculate the mean O.D. value for each standard ａnd sample. All O.D. Values are6 subtracted by the mean value of the balnk well before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis ａnd extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve. 5. Intra-assay CV(%) is less than 10% ａnd Inter-assay CV(%) is less than 15%. 6. Assay range: 1.25 ng/mL – 40 ng/mL. 7. Sensitivity: The minimum detectable dose of Mouse Aβ1-42 is typically less than 0.1 ng/mL. 8. Cross-reactivity: This assay recognizes recombinant ａnd natural Mouse A β 1-42. No significant cross-reactivity or interference was observed. 9. Storage: 2-8℃ (Use frequently); six months (-20℃)。 10. Standard curve
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